Enzymatic protein hydrolysates from aduwa ( Balanities Aeqyp | 88945
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African Journal of Food Science and Technology

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Enzymatic protein hydrolysates from aduwa ( Balanities Aeqyptiaca L) seed meal supplemented diet on α-amylase, α-glucosidase and anti-oxidants activity of Streptozotocin-induced diabetic wister albino rat


Ogori A Friday*, Girgih T Abraham, Eke M Ojotu, Abu J Oneh, Famuwagun A Albert and Adefegha S Adeniyi

The Aduwa seeds were toasted, milled, and defatted. The defatted meal is used to produce protein isolate. The Aduwa enzymatic hydrolysed peptides were produced from Aduwa protein isolate using pancreatin, and pancreatic-pepsin enzymes combination. The work investigated the anti-diabetic and anti-oxidant effects of Aduwa enzymatic hydrolysed peptides supplemented diet in streptozotocin (STZ)-induced diabetic rats. The rats were given high fat diet (HFD) for two weeks and made diabetic by intra peritoneal administration of STZ (35 mg/kg b.w.) and fed diets containing 5% (APHpa) and 5% (APHpa + pe) for 14 days. The effects of the diet on blood glucose, α-amylase, α-glycosidase, and anti-oxidant activities were monitored. Marked increases in the blood glucose and thiobarbituric acid reactive substances (TBARS); with corresponding decrease in feed intake weight gain, fed conversion ratio, α-amylase and intestinal α-glucosidase inhibitory activities and anti-oxidant capacity were monitored in diabetic rats. These trends were however reversed in diabetic rats fed diets supplemented with Aduwa protein hydrolysates of APHpa and APHpa+pe for 14 days. The Aduwa Protein hydrolysates were able to attenuate starch digestion, absorption and anti-oxidant capacity in serum hydrolysate supplemented diet. Thus, these anti-diabetic properties and anti -oxidant capacities from Aduwa supplemented diet may be attributed to the influence of its constituent phytochemicals and peptides inherent in hydrolysates supplemented diet.

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