Ren Peng, Jinping Li, Dongzhi Wei
Flavor esters are an important kind of flavor compounds, which are widely used in the food and pharmaceutical fields. The recombinant CS-2 lipase was immobilized after purification for which kieselguhr showed 18.5% of binding efficiency with 150.7 U/g of lipase activity. The immobilized lipase was used for the synthesis of butyl acetate in n-heptane. The factors affecting the synthesis of butyl acetate were investigated in the study. The results showed that the conversion of 98.2% was achieved under the following condition: reaction time (10 h); water activity (0.02); reaction temperature (55â�?�?); the concentration of acetic acid and n-butanol (0.1 mol/L and 0.2 mol/L); the addition of 4 Å molecular sieve (0.5 g) at 8h of reaction time. The conversion of substrate decreased only from 98.2% to 87.4% after five cycles in use of the immobilized lipase.
Share this article