International Research Journals

Asif Hanif

Department Of Health and Clinical Research, Pakistan


  • Mini Review   
    Differentiation of Human Induced Pluripotent Stem Cells from Patients with Severe COPD into Functional Airway Epithelium
    Author(s): Asif Hanif*

    Chronic Obstructive Pulmonary Disease (COPD), a main reason of mortality and disability, is a complicated sickness with heterogeneous and ill-understood organic mechanisms. Human triggered pluripotent stem cells (hiPSCs) are a promising device to version human sickness, which includes the effect of genetic susceptibility. Methods: We evolved an easy and dependable approach for reprogramming peripheral blood mononuclear cells into hiPSCs and to distinguish them into air–liquid interface bronchial epithelium inside forty five days. Importantly, this approach does now no longer contain any mobileular sorting step. We reprogrammed blood cells from one wholesome manipulate and 3 sufferers with very extreme COPD. The suggest mobileular purity on the definitive endoderm and ventral anterior foregut endoderm (vAFE) ranges was >80%, assessed with the aid of using quantifying C-X-C Mot.. View More»
    DOI: 10.14303/irjbcs.2022.18

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