Human Lung Adenocarcinoma-Derived A549 Clones: Gene Expressi | 93090
International Research Journals

International Research Journal of Biochemistry and Bioinformatics

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Human Lung Adenocarcinoma-Derived A549 Clones: Gene Expression


Mariya Daniel*

The GenSensor Suite consists of four online applications to elucidate connections between genes and proteins. When compared to a background list, GenPath's results show if certain categories of biochemical, regulatory, or other gene sets are over- or underrepresented in an input list. Along with all of the usual gene sets that may be searched, GenPath also provides several unique gene sets. Users can add original backdrop lists. GenPath is used to assess the interaction gene list that GenInteract generates from a single input gene. GenPubMed uses a PubMed query to locate the collection of PubMed IDs in order to derive a gene list from it and query it in GenPath (Kondo H et al., 2015). One gene set can be compared to another in GenPath by GenViewer users. Stem cell therapy appears to have promise for regenerating damaged or irreparable lung tissue. It is difficult to create a simple and reproducible approach for lung progenitor populations since the molecular process driving differentiation of alveolar epithelial cells is not fully understood. We investigated an in vitro system to evaluate the regulation mechanisms of alveolus-specific gene expression using the human alveolar epithelial type II cell line A549. Following the cloning of A549 subpopulations, each clone was separated into five groups based on the cell shape and marker gene expression. Two clones, B7 and H12, received more testing. Both H12 and B7 grew more of the ATII marker surfactant protein C when cultivated in a serum-free environment. Aquaporin 5 (AQP5), an ATI marker, was significantly upregulated in B7 and H12, respectively (Mikkonen L et al., 2010).

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