Ngueguim Tsofack Florencea, Dzeufiet Djomeni Paul Desirea, Donfack Jean Hubertb, Ngo Lemba Esthera, Tsala Emery Davida, Renaud Beauwensc, Kamtchouing Pierrea and Dimo ThÃÆÃÂ©ophile
This study was undertaken to determine the in vivo and in vitro antioxidant effect of methanol-derived extract from Dorstenia picta twigs. Diabetes was induced by streptozotocin injection in male wistar rats. After two weeks, treatment with either Dorstenia picta plant extract or insulin was initiated. Antioxidant activities of the plant extract were investigated by evaluating the levels of malondialdehyde, reduced glutathione and some antioxidant enzymes such as superoxide dismutase and catalase. In addition β- carotene linoleic acid model system was used for the evaluation of the in-vitro antioxidant activity and the total phenolics content of the plant extract was also estimated. Daily administration of methanolderived extract from D. picta during 14 days significantly decreased the blood glucose levels. The treatment indicates the reduction of lipid peroxidation and the improvement of antioxidant parameters. We found that, in comparison with untreated diabetic control rats, oral administration of methanolderived extract from D. picta at the dose of 150 mg/kg induced a significant decrease in lipid peroxidation, catalase and superoxide dismutase activities with an increase in glutathione level. In-vitro study showed that, the extract contains 16.77 GAE/μg of total phenolics and possess antioxidant activity with IC50 value 32.26 ±1.27 μg/mL in β-carotene bleaching inhibition assay. These results indicate that methanol-derived extract from D. picta twigs controls glycaemia, improves the oxidative status thus decrease the damage caused by oxidative stress in diabetic rats.
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