GET THE APP

International Research Journals

Research Article - International Research Journal of Plant Science ( 2022) Volume 13, Issue 6

Basic studies on cyanobacteria and Micro algae: A Review

Radhika Ikkurti* and Ch. Arathi
 
G.Narayanamma Institute of Science & Technology, Shaikpet, Hyderabad, India
 
*Corresponding Author:
Radhika Ikkurti, G.Narayanamma Institute of Science & Technology, India, Email: daakshayini.radhika@gmail.com

Received: 06-Dec-2022, Manuscript No. IRJPS-22-72822; Editor assigned: 09-Dec-2022, Pre QC No. IRJPS-22-72822; Reviewed: 23-Dec-2022, QC No. IRJPS-22-72822; Revised: 27-Dec-2022, Manuscript No. IRJPS-22-72822; Published: 30-Dec-2022, DOI: http:/dx.doi.org/10.14303/irjps.2022.037

Abstract

Cyanobacterial lipids and profiling have become a tremendous demand due to its applicability for production of biofuels. They also act as maker for identification of isolates. Cyanobacteria possess different photosynthetic pigments like chl-a, phycobili proteins and carotenoids which have substantial commercial applications. Phycobili proteins are the major photosynthetic adjunct pigments of cyanobacteria.

Keywords

Phycobilli, Cyanobacteria, Algae.

Introduction

Chlorophyll –a is a pigment commonly found in cyanobacteria that participates directly in the light requiring reactions of photosynthesis. Chlorophyll-a has one of the functional groups bonded to the porphyrin (CH3 group). Chlorophyll a is a large molecule that has a “head” called a porphyrin ring with a magnesium atom at its center. Chl-a is generally used as an index of phytoplankton biomass (Falkowski et al., 1998). The phycobiliproteins have been classified into several groups’ viz. phycoerythrin, phycocyanin and allophycocyanin. Phycocyanin and allophycocyanin are universally present in cyanobacteria, while allophycocyanin B apparently occurs in most cyanobacteria (Glazer & Bryant, 1975; Swings & De Ley J, 1977). Many reports are available on the importance of carotenoids related to prevention of health disorders (Moeller et al., 2000). They are also being used as a natural colorant and for cosmetic properties (Cohen et al., 1988). It’s Collection, Isolation and Identification of Cyanobacteria has been well explained in a paper (Singh et al., 2022). Simple plants which lack root, stems and leaves; mainly aquatic thallophytes having Chl-a as primary photosynthetic pigment is considered as algae, commonly called blue-green algae. It has been evolved about 3.5 billion years ago.

It Has Chl-a, (some also have b or d), phycobili proteins, glycogen as storage products. Its Cell wall containing amino sugars and amino acids which involves in Oxygen evolving photosynthesis.

The identification of the taxa was done by observing the morphological features and comparing these features with the literature. Desikachary, 1959; Komarek and Anagnostidis, 2005 (Figure 1).

plant-science-taxa-literature

Figure 1. Showing identification of the taxa literature.

Features of Cyanobacteria: The Important features include filaments shape; cell dimensions; presence/absence of sheath, thickness; shape, size and position of akinetes/heterocyst, if present etc. were observed under the microscope.

Importance and Industrial Value of Cyanobacteria

Agriculture: Biocontrol agents, Novel gene pool for crop improvement, Development of sustainable agriculture and ecosystem, Biofertilizer, Plant growth promoter (Figure 2).

plant-science-taxa-literature

Figure 2. Agricultural importance of cyanobacteria.

Food: Spirulina has been eaten for centuries by Kanembu people, who live along the shores of Lake Chad in northcentral Africa.It has the highest protein of any natural food (65%).It is of current world algal production, 30% is sold for animal feed applications and over 50% of Spirulina is used as feed supplement (Figure 3).

plant-science-taxa-literature

Figure 3. Cyanobacteria used as food.

Spirulina is used as feed in the diet of sheep, horses, Canary, finch, parrot, love bird. It is used by ostrich and turkey breeders to increase fertility.

Species of Nostoc, Anabaena and Calothrix increase body weight of Telapia hybrid fish with excellent food conversion ratio.

Pigments and Natural Colours: Apart from chlorophyll, cyanobacteria produce two major groups of pigments (phycobiliproteins and carotenoids).From them; Phycocyanin and Phycoerythrin are commercially valuable.

Phycocyanin has been extracted and purified from Spirulina sp., Synechococcus sp., Oscillatoria quadripunctulata, Aphanizomenon flosaquae, Anabaena sp. Dainippon Ink & Chemicals (Sakura, Japan) has developed a product called “Lina blue” (PC extract from Spirulina platensis), which is used in chewing gum, candies, soft drinks, dairy products (Figure 4).

plant-science-taxa-literature

Figure 4. Use of Phycobilins in cosmetics (lipstick, eyeliners etc.) is also gaining momentum.

Medicinal Properties: Phycocyanin has also been reported to reduce the levels of tumor necrosis factor (TNF-a) in blood serum of mice treated with endotoxin. Phycocyanin from Aphanizomenon flos-aquae is reported as a strong antioxidant. Carotenoids present in CB protect cells from damage by reactive oxygen molecules, hence, act as antioxidants. Spirulina, a rich source of b-carotene, has been used in aquaculture and poultry to improve colour of fishes and egg yolks respectively. Chlorophyll-a extracts from Spirulina having iron oxide and higher alcohols (cetyl and stearyl) is patented as strong deodorant (Figure 5).

plant-science-taxa-literature

Figure 5. Phycocyanin is able to scavenge alkoxyl, hydroxyl and peroxyl radicals in vitro.

Cosmetics: A protein-rich extract from Spirulina platensis repairs signs of early skin aging, exerts a tightening effect and prevents stretch mark formation (Protulines, Exsymol S.A.M., Monaco). Spirulina Firming Algae Mask by ODA (Optimum Derma Aciditate) improves moisture balance of skin.

Spirulina Whitening Facial Mask by Ferenes cosmetics contains proteins from Spirulina and herbal extracts, which improve skin complexion, and reduce wrinkles without any allergic effect. Cosmetics contain quality ingredients from Spirulina and other herbs that remove dead skin cells and act as a cleanser to energize the face (Figure 6).

plant-science-taxa-literature

Figure 6. Spirulina Facial Scrub by Ferenes.

Antibiotics & Drugs: BGA like Anabaena, Nostoc and Oscillatoria produce a great variety of secondary metabolites. Nostoc species were used to treat gout, fistula and several forms of cancer. Noscomin, a diterpenoid from N. commune, showed antibacterial activity against Bacillus cereus, Staphylococcus epidermidis, and Escherichia coli. Natural products of Nostoc sp. are effective against Cryptococcus sp. as a causal agent of secondary fungal infections in patients with AIDS. Fibre extracted from N. commune, possesses hypocholesterolemic effect in rats.Lyngbya majuscula, a marine CB, and produces a wide variety of polyketides, lipopeptides, cyclic peptides and many others (Figure 7).

plant-science-taxa-literature

Figure 7. Antibiotics product.

Fischerella ambigua and Haplosiphon hybernicus produce Ambiguines, which show anti-fungal activity on Aspergillus oryzae and Candida albicans. An anti-cancer factor has been identified in Scytonema sp., Phormidium tenue and Anabaena variabilis.

Enzymes: Microcystis aeruginosa possesses a large capacity to mineralize organic per unit of biomass due to alkaline phosphatase activity. A number of studies report detection of enzymes (phosphatase, arylsulfatase, chitinase, L-asparaginase, L-glutaminase, amylase, protease, lipase, cellulase, urease and lactamase) produced by CB. An anti-fertility enzyme, elastase, has been purified from Oscillatoria sp.

Biofuels Some unicellular non-diazotrophic CB Gloeocapsa alpicola (shows increased H2 production under sulphur starvation), Spirulina platensis (can produce 1 mmole H2/12 h/mg cell dry wt in complete anaerobic and dark condition), diazotrophic CB Anabaena variabilis etc. are reported for H2 production.

Bioremediation: Phormidium ambiguum and Chroococcus minutus have the efficiency to remove lignin from the waste waters of the paper mills (Bharati et al., 1992). Phormidium valderianum BDU 30501 is able to remove and degrade phenol (Shashirekha et al., 1997). Fifteen strains of cyanobacteria including Anabaena sp. PCC7120, Nostoc ellipsosporum are known to degrade lindane (Kuritz 1999). Coir waste containing cellulose and lignin content can be degraded by three marine cyanobacterial strains namely Phormidium sp., Oscillatoria sp. and Anabaena azollae sp. having different rate of degradation (Anbuselvi & Rebecca 2009).

Negative Value: The contaminate water supplies by producing colour, odour and giving a fishy taste to the drinking water. Growth can be checked by addition of very small quantities of copper sulphate and dichlorophen to the water, Chacko (1970) reported that thick blooms caused by Microcystis, Nostoc, Anabaena, Oscillatoria and some other BGA bring about depletion of oxygen resulting in large scale death of fish and other aquatic animals (Figure 8).

plant-science-taxa-literature

Figure 8. Negative effects on aquatic animals.

Preparation methods: There are many methods of molecular extraction for cultured algae and they include, DNA extraction, purification and gel loading Cells are usually cultured in liquid medium and then collected by centrifugation .This process is called as Lyophilization. After this purification of amplified products takes place and we get Cyanobacteria, Eukaryotic algae with their base pair.

Further sequence the product accordingly to set reaction for PCR amplified product for forward and reverse primer. Thereafter isolate intact total RNA with minimal contamination from DNA, proteins and lipids (ultimate goal to study the level of gene expression) which also involves RNA gel electrophoresis.

Conclusion

There are many standardization techniques for Eco friendly cultivation at low cost cultivation, processing of pharmacologically active traditional and aromatic plants which we will be discussing in our next paper.

Acknowledgements

Authors sincerely thank G. Narayanamma Institute of Technology & science for their encouragement, and Cooperation for publishing this paper.

References

Anbuselvi S, Rebecca J (2009). A comparative study on the biodegradation of coir waste by three different species of Marine cyanobacteria. J Appl Sci Res. 5: 2369-74.

Google Scholar

Bharati SG, Salanki AS, Taranath TC, Acharyulu MV (1992). Role of cyanobacteria in the removal of lignin from the paper mill waste waters. BECT. 49: 738-42.

Indexed at, Google Scholar, Cross Ref

Cohen Z, Vonshak A, Richmond A (1988). Effect of environmental conditions on fatty acid composition of the red alga Porphyridium cruentum: Correlation to growth rate 1. J Phycol. 328-332.

Indexed at, Google Scholar, Cross Ref

Desikachary TV (1959). Cyanophyta. New Delhi: Indian Council of Agricultural Research.

Google Scholar

Falkowski PG, Barber RT, Smetacek V (1998). Biogeochemical controls and feedbacks on ocean primary production. Science. 281: 200-206.

Indexed at, Google Scholar, Cross Ref

Glazer AN, Bryant DA (1975). Allophycocyanin B (λmax 671, 618 nm). Arch Microbiol.104:15-22.

Indexed at, Google Scholar, Cross Ref

Komarek J, Anagnostidis K (2005). Subwasserflora von Mitteleuropa: Cyanoprokayota 2. Oscillatoriales. 1-759.

Google Scholar

Kuritz T (1999). An easy colorimetric assay for screening and qualitative assessment of deiodination and dehalogenation by bacterial cultures. Lett Appl Microbiol. 28: 445-447.

Indexed at, Google Scholar, Cross Ref

Moeller SM, Jacques PF, Blumberg JB (2000). The potential role of dietary Xanthophylls in cataract and age-related macular degeneration. J Ame Coll Nutri. 19:522S-7S.

Indexed at, Google Scholar, Cross Ref

Shashirekha S, Uma L, Subramanian G (1997). Phenol degradation by the marine cyanobacterium Phormidium valderianum BDU 30501. J Ind Microbiol Biotechnol. 19: 130-133.

Indexed at, Google Scholar, Cross Ref

Singh Y, Singh G, Singh DP, Khattar JI (2022). A checklist of blue-green algae (Cyanobacteria) from Punjab, India. J Threat Taxa. 14: 20758-20772.

Indexed at, Google Scholar, Cross Ref

Swings J, De Ley J (1977). The biology of Zymomonas. Bacteriol Rev. 41:1-46.

Indexed at, Google Scholar, Cross Ref

Citation: Ikkurti R (2022). Basic studies on cyanobacteria and Micro algae: A Review. IRJPS. 13: 037.