Umbilical Cord Stem Cells\' Osteogenic Differentiation And M | 95612
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Umbilical Cord Stem Cells\' Osteogenic Differentiation And Mechanical Properties As A Result Of Electrospun Submicron Fibers In A Calcium Phosphate Cement Scaffold


Alok Ranjan*

In the bone defect, calcium phosphate cements (CPCs) can be injected and self-set. There are no reports of stem cell seeding on CPCs with Electrospun submicron fibers, according to a literature search. For the first time, this study wanted to find out how Electrospun fibers in CPC affected mechanical properties as well as the proliferation, osteogenic differentiation, and mineralization of human umbilical cord mesenchyme stem cells (hUCMSC). Electro spinning was used to produce poly (PLGA) fibers with an average diameter of 650 nm. Tetra calcium phosphate, dicalcium phosphate anhydrous, and chitosan lactate were the fibers that were included in CPC. There were 0%, 2.5%, 5%, and 10% fiber volume fractions. Work-of-fracture (toughness) and flexural strength of CPC with 10% fibers were twice as high as those of CPC without fibers. While attaching to the Electrospun fiber-CPC scaffolds multiplied rapidly and produced bone minerals. When the fiber volume fraction in CPC was increased from 0% to 10%, the expressions of alkaline phosphatase, osteocalcin, and collagen I in hUCMSC were doubled, and mineralization was also increased by 40%. The fiber-CPC scaffold's high surface area and biomimetic properties were blamed for the improved cell function. In conclusion, the incorporation of submicron fibers into CPC significantly enhanced its toughness and strength.

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