Gembong Satria Mahardhika
The emergence of strains of bacteria that are resistant to many antibiotics including Staphylococcus aureus bacteria, is a serious problem, moreover it is supported by the ability of these bacteria to form biofilms, causing antimicrobial agents and immune system responses are not effective in eliminating biofilm cells. The aim of this study is to understand the comparison of ica A/D genes in MRSA producing biofilms between health workers in hospitals and the general population in Banyumas. This study is a cross sectional study aimed at describing the ica A/D gene in biofilm-producing MRSA among health workers in TK Wijayakusuma Hospital compared to the general population in Banyumas. Samples were taken using the nasal swab method. Microbiological examination was carried out to identify MRSA strains. Biofilm identification uses a microtiter plate biofilm assay, after that it detects the ica A/D genes. A number of 120 samples were examined, one sample (0.008%) showed MRSA results and 6 samples (0.05%) showed MSSA results. A number of 7 samples were examined for biofilm showing one sample with moderate biofilm results, 2 weak biofilm samples, 4 non biofilm samples. Examination of ica A/D gene was performed on 3 samples that showed moderate biofilm and weak biofilm results. The results in 3 samples showed positive ica A/D gene results. Comparison between ica A/D genes in health workers in hospitals with the general population cannot be done, because MRSA samples are only found in the health care group in hospitals. The conclusion from this study is there was no difference in the ica A/D gene in MRSA producing biofilms between health workers in the hospital compared to the general population in Banyumas district, because only one MRSA sample was found, that was in the group of health workers.
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