Massimo Giangaspero, Claudio Apicella, Ry̮̫ Harasawa
The genus Pestivirus of the family Flaviviridae is represented by four established species; Bovine viral diarrhea virus 1 (BVDV-1), Bovine viral diarrhea virus 2 (BVDV-2), Border disease virus (BDV) and Classical swine fever virus (CSFV) and a tentative ��?Giraffe� species. The palindromic nucleotide substitutions (PNS) in the 5��? untranslated region (UTR) of Pestivirus RNA have been described as a new, simple and practical method for genotyping. The preparation of specific software was considered for an easy access of the users to the method. In the present study, the new software, named PNS, was prepared and the application on the genotyping procedures with the keys for Pestivirus identification as specific PNS at genus, species and genotype level, respectively, was evaluated on five hundred-tirty-four sequences. The software is freely available at www.pns-software.com
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