International Research Journal of Pharmacy and Pharmacology

New and sensitive spectrophotometric method for determination of xipamide in pure and dosage forms by complexation with Fe(III), Cu(II), La(III), UO2(II), Th(IV) and ZrO(II) ions

Abstract

Mohamed Gaber, Abdalla M. Khedr, Ahmed S. El-Kady

A new, rapid, sensitive and selective method for the spectrophotometric determination of xipamide in pure and dosage forms is proposed. The method is based on the reaction ofxipamide with Fe(III), Cu(II), La(III), UO2(II), Th(IV) and ZrO(II) ions to form stable coloredcomplexes. The formed complexes have maximum absorbance at 500, 390, 335, 445, 325 and 333 nm for Fe(III), Cu(II), La(III), UO2(II), Th(IV) and ZrO(II), respectively. Optimumconditions for determination, stoichometry and stability of the formed complexes have beendetermined. Beer's law is obeyed up to concentration range 54.64-1995.75 mg ml-1 of xipamide with apparent molar absorptivities ranging from 0.43 x 103-7.20 x 103 l mol-1 cm-1. The proposed method has been applied successfully for the determination of xipamide in pure form and representative pharmaceutical formulations with high precession and good accuracy.  

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