Investigations on embryos and seeds are very difficult, owing to the fact that orchid seeds are very small, dusty, and transparent. Therefore, this study looked at how to prepare the maceration technique for orchid seeds and how to isolate orchid embryos from seeds. The seeds of Barlia robertiana (Orchidaceae) were used. Consequently, for orchid seeds, the most suitable maceration method was established and its practical importance is discussed.
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