M.A. Badhul Haq, D. Mohammed Azarudeen, R. Vignesh, T.T. Ajith Kumar and M. Srinivasan
Efficiency of barcoding is decisively dependent upon species description, splitting decreases while the protuberance increases both intraspecific variation and interspecific divergence. In the present study T.blochi (silver pompano) was sequenced for its 700 bp region of cytochrome oxidase subunit I (COI) gene to test its efficacy in identifying the species and also to demonstrate its intra species variations within the barcode region. The present study addresses this issue by examining the patterning of COI diversity in the subphylum Vertebrata, the most ancient and structurally diverse group of family Carangidae. The sequences were analyzed for their species identification using Barcode of life database (BOLDâ�?�?s) identification engine. The BOLDâ�?�?s identification engine was also used to draw species level phylogenetic tree. The COI sequences of T.blochi from different geographical regions were extracted from NCBI for intraspecies variation analysis. All sequences were aligned using Clustal W. Phylogenetic tree was constructed with bootstrap test with 500 replicates. The barcode profiling studies clearly revealed that the barcode region of T.blochi from different waters had high cytosine content whereas guanine content was found least. Interesting results were obtained in case of hypothetical barcode protein profiling, as the percentage of leucine was found higher in barcode proteins.
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