The present research was aimed to formulate the herbal sanitizer, from Neem, Lemon juice and Tulasi. Phytochemical properties of neem extract, lemon juice, juice and Tulasi were analysed. Their zone of inhibition was checked against standard culture. Zone of inhibition of all the formulations was found. Formulated sanitizer was compared with sterillium sanitizer and it was found that sterillium was effective against E. coli organism. Hand sanitizers were effective against the test organism. The antimicrobial effectiveness was assessed by measuring the zone of inhibition against the test organism. Maximum inhibition (in mm) was seen in group A (Sterillium), i.e., 10.5 ± 0.707 and minimum in group B (Tulasi), i.e., .0 ± 0.707. The difference in the values of the different sanitizers was statistically significant. From the present study, it was concluded that sterillium was most effective and sanitizers prepared from herbal extracts was close and comparable.
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