Evaluation of physiological, biochemical and enzymatic activ | 16617
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International Research Journal of Biotechnology

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Evaluation of physiological, biochemical and enzymatic activity during accumulation of CoCl2 in Cyamopsis tetragonoloba (L.) TAUB and its impact on protein profile


P. Gurusaravanan, S. Vinoth, Roy John, G. Siva, N. Boopala Bhagavan, V. Pandiyarajan and N. Jayabalan

We investigated the changes in the morphology, biochemical, antioxidant enzyme activity and protein profiles in Cyamopsis tetragonoliba (L). The crop was treated with different concentrations of CoCl2 (25, 50, 75, 100, 125 µM-1) and the changes in growth, protectively induced oxidative stress in relation to metal stress after 14 days old plant were examined. In this study, the lower concentration of 25 µM cobalt chloride has increased the plant productivity than the other concentrations tested. Chlorophyll and carotenoid pigments decreased significantly in the stressed leaves for their adaptation in stressed condition. The two isozymes CAT 1 and CAT 2 activity were detected from the extract of treated and stress leaves were subjected to polyacrylamide gel electrophoresis. Most prominent peroxidase enzymes were induced by accumulation CoCl2 and possibly resulting in improved effectiveness of the enzyme at high concentration of stressed leaves. The protein profile of cluster bean (Cyamopsis tetragonoliba L.) strongly influenced by severe heavy metal stress (100 µM and 125 µM). It was analyzed through SDS-PAGE and found four new polypeptides, hence it was concluded that more number of new proteins (190 kDa, 54 kDa, 38 kDa and 33 kDa) were synthesized in stress treated plants and it was highly intensive and probably constitutes stress tolerant proteins.

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