Arcego, D.M., Noschang, C., Krolow, R., Fitarelli, L.D., Laureano, D., Huffell, A.P., Fontella, F.U., Dalmaz, C.
Early life events lead to a large number of behavio ral and biochemical alterations in adulthood. The aim of this study is to verify whether the release of gonadal hormones during puberty affects parameters of oxidative stress observed in adulthoo d in cerebral cortex, striatum and hypothalamus of female rats subjected to neonatal handling. Rats we re exposed or not to neonatal handling (10min/day, first 10 days of life). Between 21-28 post-natal da ys, females from each litter were divided into the following groups: ovariectomy, sham, and intact (no surgery) when adults, parameters of oxidative stress were analyzed. The groups subjected to surger y (ovx and sham), showed increased production of free radicals by the method of oxidation of dich lorodihydrofluorescein (DCFH) in cerebral cortex an d striatum. Decreased catalase activity was observed in the cerebral cortex, in the same groups. No effects of neonatal manipulation were observed in t hese structures. We conclude that the period after weaning constitute a critical window for stressful interventions during development, leading to alterations in parameters of oxidative stress in ad ulthood, and these effects are not influenced by estradiol and neonatal handling.
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