Full Length Research Paper
Chronic chlorpyrifos-induced sensorimotor and cognitive deficits in Wistar rats- Reparation by Vitamin C
Suleiman F. Ambali1,2, Joseph O. Ayo3, Samuel A. Ojo4 and King A. N. Esievo5
1Department of Veterinary Physiology and Pharmacology, University of Ilorin, Ilorin, Nigeria
2Department of Veterinary Pharmacology and Toxicology, Ahmadu Bello University, Zaria, Nigeria
3Department of Veterinary Physiology, Ahmadu Bello University, Zaria, Nigeria
4Department of Veterinary Anatomy, Ahmadu Bello University, Zaria, Nigeria
5Department of Veterinary Pathology, Ahmadu Bello University, Zaria, Nigeria
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Received 30 July, 2012; Accepted 10 September, 2012
The objective of the present study was to evaluate the role of oxidative stress and mitigating effect of vitamin C on sensorimotor and cognitive changes induced by chronic chlorpyrifos (CPF) exposure in male Wistar rats. Twenty adult male Wistar rats divided into 4 groups of 5 animals each served as subject for this study. Group I (S/oil) was administered with soya oil (2 ml/kg) while group II (VC) was given vitamin C (100 mg/kg); group III was dosed with CPF (10.6 mg/kg~1/8th of the LD50); group IV was pretreated with vitamin C (100 mg/kg) and then exposed to CPF (10.6 mg/kg), 30 min later. The regimens were administered orally by gavage once daily for a period of 17 weeks. The animals were evaluated for toxic signs and neurobehavioral parameters. At the end of the period, the whole brain samples were evaluated for the levels of malonaldehyde (MDA) and activities of superoxide dismutase (SOD), catalase (CAT), and acetylcholinesterase (AChE). The result showed that toxic signs and the impairments of coordinated gaits, neuromuscular coordination, learning and short-term memory induced by CPF exposure were mitigated by vitamin C. The increased MDA concentration in the CPF group was significantly reduced by vitamin C pretreatment. The activities of SOD, CAT and AChE which were reduced by CPF exposure significantly improved in vitamin C pretreated group. In conclusion, this study has shown that vitamin C mitigates sensorimotor and cognitive deficits induced by chronic CPF exposure in Wistar rats apparently due to its AChE restoration and antioxidant properties.
Keywords: Chlorpyrifos, Neurobehavioral deficit, Oxidative stress, Acetylcholinesterase, Antioxidant, Reparation, Vitamin C.