International Research Journals

International Research Journal of Biochemistry and Bioinformatics

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Xavier Elena

Department of Chemical and Biomolecular Engineering, USA


  • Review Article   
    Searching for early-onset protein aggregation to identify longevity mutations
    Author(s): Xavier Elena*

    Genetic screening techniques are frequently employed to find the genes responsible for particular biological processes. Forward genetic screens in Caenorhabditis elegans rely on the discovery of mutants that are capable of self-propagating clonal colonies and are thus reproductively active (Zhou et al., 2014). Thus, it is difficult to develop screens that focus on post-reproductive characteristics, including longevity. We accomplish highthroughput automated screening for short-lived mutants utilising protein aggregation as a marker for ageing using microfluidic technology and image processing. Using fluorescently-labeled PAB-1 as a readout, we use microfluidics to undertake repetitive high-throughput analysis and sorting of animals with enhanced protein aggregation as well as to maintain a reproductively active adult mutagenized population (Hermann et al., 1999). We show that longevit.. View More»
    DOI: 10.14303/2250-9941.2022.40

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