Mini-Review - International Research Journal of Microbiology ( 2023) Volume 12, Issue 3
Received: 01-May-2023, Manuscript No. IRJM-23-98143; Editor assigned: 03-May-2023, Pre QC No. IRJM-23-98143 (PQ); Reviewed: 17-May-2023, QC No. IRJM-23-98143; Revised: 19-May-2023, Manuscript No. IRJM-23-98143; Published: 26-May-2023, DOI: 10.14303/2141-5463.2023.36
The main bioactive component of Capsicum plants is dihydrocapsaicin, which is used as a food drug and additive in China and India. In this study, four in vitro cultivated human intestinal fungal strains were used to perform the biotransformation of dihydrocapsaicin. Eight metabolites, including seven beforehand undescribed metabolites (1 and 38) and one known simple, were acquired. To determine their structures, numerous spectroscopic data, including NMR and HRESIMS, were collected. The most important biotransformation reactions were identified as hydroxylation, alcohol oxidation, and lactylation based on the structures of the dihydrocapsaicin metabolites. Specifically, the lactylation of hydroxyl bunches is for the most part interceded by Rhizopus oryzae R2701
Dihydrocapsaicin, Intestinal fungal strains, Spectroscopic, Biotransformation, Hydroxylation
Capsaicin and dihydrocapsaicin are widely recognized as the primary constituents of Capsicum plants, including hot peppers. Dihydrocapsaicin applies anticancer exercises against different harmful growths, like bosom disease, colorectal malignant growth, and glioma. Capsaicin and its metabolites have recently been found to be effective inhibitors of LSD1. The fact that dihydrocapsaicin derived from a natural product can be utilized in the development of new LSD1 inhibitors has piqued our interest since we discovered that dihydrocapsaicin is also a natural LSD1 inhibitor with an inhibition of 76.5 percent at 10 M in this study. Biotransformation has been widely considered on the grounds that it has been distinguished as a successful strategy for changing over abundant or inexpensive natural mixtures into in any case scant or exorbitant analogs. Due to their similar structures, biotransformation products of the capsaicin side chain appear to have comparable biological activities to capsaicin. Dihydrocapsaicin was biotransformed by human intestinal fungi in order to find more bioactive LSD1 inhibitors. This led to the discovery of eight metabolites, including seven new metabolites and a known analog (Stazi G, 2016). In addition, the inhibitory effect of metabolites on LSD1 was examined. Strikingly, metabolite 1 showed critical inhibitory movement against LSD1, with a restraint pace of 80.7%, which was more grounded than that of dihydrocapsaicin. Besides, metabolite 1 showed most grounded inhibitory impact on LSD1 (IC50 1.99 μM). In this study, LSD1 was found to be a target of metabolite 1 for the first time. This can serve as a new scaffold for improving the LSD1 inhibitor further. In this, we report subtleties of the biotransformation methodology and theoretical biotransformation pathway of dihydrocapsaicin and the disconnection, structure clarification, and inhibitory impact on LSD1 of the metabolites (Huang Y, 2012) (Lim S et al., 2009).
We looked at how dihydrocapsaicin and its metabolites 18 inhibited LSD1. Accordingly, the inhibitory effects of the greater part of the metabolites against LSD1 were viewed as in the scope of 25-60% at 10 μM. Quite, metabolite 1 and dihydrocapsaicin shown huge inhibitory impact on LSD1, with hindrance of 80.7% also, 76.5% at 10 μM, individually (Liang Y, 2009). In addition, the LSD1 inhibitor metabolite 1 had an IC50 value of 1.99 M. Strangely, metabolite 1 showed a higher LSD1 hindrance rate than dihydrocapsaicin, in spite of the fact that they contrasted from one another; just C-9 and C-50 in dihydrocapsaicin were both supplanted by hydroxyl gatherings (Sakane N et al., 2011) (Musri MM et al., 2010). However, the specific mechanism of this result remains a mystery and requires additional investigation. There have been numerous reports of reversible and irreversible LSD1 inhibitors, but only a few are natural (Janzer A et al., 2012) (Libby P, 2011). For example, baicalin was viewed as the principal LSD1 inhibitor (IC50 3.01 μM), and α-mangostin was viewed as the first xanthone-based LSD1 inhibitor (IC50 2.81 μM). Prominently, it has been accounted for capsaicin (IC50 0.6 μM) and capsaicin simple (9,50-dihydroxycapsaicin) (IC50 1.52 μM) both showed significant inhibitory impact on LSD1 ( Mitra R, 2022) ( Wang Yet al., 2009).
Dihydrocapsaicin was biotransformed by four intestinal fungi (Aspergillus japonicus Y4009A, Rhizopus oryzae R2701, Candida parapsilosis M8011, and Aspergillus fumigatus PB4204) into seven new metabolites and one known analog. The designs were unambiguously resolved utilizing NMR and HRESIMS spectra. The most important biotransformation reactions were identified as hydroxylation, alcohol oxidation, and lactylation based on the structures of the dihydrocapsaicin metabolites. In an in vitro bioassay, metabolite 1 had the greatest inhibitory effect on LSD1 (IC50 of 1.99 M). According to the aforementioned findings, the biotransformation of dihydrocapsaicin by intestinal fungi was an efficient strategy for the production of LSD1 inhibitors that could be utilized as treatments for cancer.