(Baumann K et al ., 2020) From leftover microalgae biomass obtained from either a lipid-based biofuel process or wastewater treatment, anaerobic digestion can be used to make methane biogas. Due of their potential for robustness in large-scale open pond production, halophytic microalgae are being considered for use in the production of biofuel. Halophytic microalgae biomass would be difficult to digest anaerobically due to high salinities that are uncommon in anaerobic digestion settings. (Austin RS et al ., 2011) As a viable substrate feedstock for anaerobic digestion, halophytic microalgae biomass would have salinities greater than 3.5%, which are frequently observed in marine environments. The first step of the study described here examines changes made in the bacterial population as a result of the anaerobic digestion of piggery effluent, which is a problem with the anaerobic digestion of halophytic microalgae. with the goal of developing a saline-tolerant anaerobic digestion inoculum capable of breaking down feedstocks under extreme salinity conditions. Positive outcomes from this inoculum development study enabled the study of halophytic microalgae's anaerobic digestion (Färe R et al., 2007). According to the results, a saline-tolerant inoculum was kept up. Following halophytic bacterial community fingerprinting using denaturing gradient gel electrophoresis (DGGE), numerous halophytic methanogens were discovered. It was the inoculum that broke down the halophytic microalgae. The obtained gas data demonstrated that at 7% salinity, biogas generation of 358 53 mL/g of volatile solids (VS) with a methane concentration of 54 4.3% was accomplished. Wet weight microalgae biomass produced 122 26 and 175 25 mL/g of biogas, respectively. of halophytic microalgae biomass (74 2.8 wt.% moisture content), respectively. At salinities of 3.4% and 7%, respectively, the conversion of carbon in the feedstock to methane was successful with efficiencies of 26.4% and 46.6%. In order to close the loop on nutrient recycling needed for the generation of halophytic microalgae based biofuels and maybe, hypersaline wastewater treatment applications, a halo-tolerant anaerobic digestion microbial community could be further developed (Dagum C et al., 1997).
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