Ni Made Suaniti, A A Gede Sudewa Djelantik, Ketut Suastika, I Nyoman Mantik Astawa
To obtain an exact analytical method development va lidation should be performed to verify that the parameters are quite able to overcome the problem o f performance analysis. Analysis of the chemical after drinking ethanol can be determined by analysi s of ethanol in biological fluids such as blood, th e next in a long time can not be detected because eth anol can form other metabolites. One of the metabolites that can form is Fatty acid ethyl ester s (FAEEs) because ethanol reacts with fatty acids t o produce neutral molecules known as esters. FAEE as a biomarker of ethanol is more sustainable than ethanol itself to analyse at any given time. The pu rpose of this experimental study is to examine the significance biochemical markers of alcohol given b y mouth in the Wistar rats. The study design use "True randomize experimental post test only control group design." The rats are randomly distribute according to experimental design and are treated da ily for one week (acute) with 20% alcohol. This study used 10 rats; with five rats for the treatmen t group with 20% alcohol acute and 5 rats treat as control group with distill water. The first analysi s of ethanol and the second analysis of FAEE as biochemical markers of ethanol was done by gas chro matography. Blood samples are collected at 6 and 24 hours after the last oral intake of acute al cohol administration. The presence of FAEE shows that is persisting longer than ethanol and analysis by non-parametric test. At 6 hours after drinking 20% ethanol treatment, FAEE is significantly less d etectable (1.8) than ethanol (3) (p <0.05) but 24 hours after drinking 20% ethanol treatment, is more detectable FAEE (2.4) than ethanol (1.2) (p < 0.05 ).
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