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Study of seed proteins pattern of brassica napus varieties v | 16323
International Research Journals
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International Research Journal of Biotechnology

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Study of seed proteins pattern of brassica napus varieties via sodium dodecyl sulfate polyacrylamid gel electrophoresis

Abstract

Mehdi Kakaei and Danial Kahrizi

Canola (Brassica napus L.) is an oilseed crop adapted to various environments. The current research was conducted to determine the seed storage protein profiles of 12 Brassica napus cultivars (Geronimo, Celecious, Milena, Sahara, Sunday, Zarfam, Dante, SLM-046, Talaye, Talent, ARC-2, Opera) that were analyzed by Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) based on biochemical markers. For this purpose total soluble proteins were resolved on 15% SDS polyacrylamide gels. Then seed protein was extracted by extraction buffer. The results of SDS-PAGE were shown 17 bands on gel that they had high polymorphism. Finally genotypes were clustered into three groups by applying un-weighed pair group mean analyses.

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