International Research Journal of Biochemistry and Bioinformatics

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Seed storage protein profiling: A method to reveal genetic variation in local land races and wild forms of mungbean

Abstract

Swapan K. Tripathy, Rajesh Ranjan, Jayashree Kar, Bhabendra Baisakh, Pramod K. Nayak, Digbijaya Swain, Gokul B. Dash, Pramod C. Satpathy and Sasmita Dash

The SDS-PAGE of 81 land races, four improved varieties and four wild forms of mungbean revealed 11 scorable polypeptide bands each of albumin and globulin seed storage protein fraction with molecular weights ranging from 6.5 to 100.2kD and 18.2 to 85.9kD respectively. Barring two monomorphic bands at 45.0kD (albumin) and 53.7kD (globulin), rest of the polypeptide bands had shown polymorphism to the extent of 90.09% among the test genotypes. Sikri local and Sidheswar local could be identified owing to unique presence of globulin bands at mol. wt position 27.5kD and18.8kD respectively. One albumin band at 48.2kD and two globulin bands at 85.9 and 18.8kD were visualized in TCR 20( wild accession) as well as in almost all local land races and cultivated check varieties, but such bands were specifically absent in all other viny wild forms. The clustering pattern revealed nine genetic clusters. Each of the viny wild forms (TCR 213, TCR 243 and TCR 192) were clearly isolated into three initial single clusters followed by two divergent phyllogenetic clusters of land races and a single cluster containing wild accession TCR 20. Thus, TCR 20 seems to have more proximity to cultivated forms than other wild accessions. Besides, a local land race, Balarampur Keonjhar local being morphologically distinct (vigorous growth, broad leaves, long pod, bold seeds) from other land races; was isolated to form single cluster. Further, it was revealed that genotypes in the same cluster based on seed protein profiling have some common agronomic performance and such clusters with unique morpho-economic traits could be identified for genetic improvement. TCR 20 and Balarampur Keonjhar local being highly divergent with high yield potential have been sorted out as valuable material for hybridization with local land races and ruling check varieties of mungbean. .

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