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International Research Journal of Microbiology

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Rotavirus infection and its monitoring in waste water using RT- PCR in Jeddah, Saudi Arabia

Abstract

Nezar A. Redwan* and Ruba M. Attar

Rotaviruses are the most common etiological agent of severe diarrhea in infants and young children Sewage systems are important nodes to monitor enteric pathogens transmitted via water. The aim of this study was to assess the presence of rotaviruses in wastewater receiving streams in Jeddah, Saudi Arabia, to provide viral fate and transport data for further epidemiological studies. In this study, one hundred of waste water samples were collected between 2009 and 2010 from the wastewater receiving outlet of AL-Misk Lake in Jeddah city. Samples were screened for the presence of rotavirus by reverse transcriptase-polymerase chain reaction (RT-PCR) technique. A total of 65 (65%) samples were found to be positive for rotavirus using this technique. The seasonal distribution of rotavirus diarrhea showed a winter peak, with an unusual peak from June to September.

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