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International Research Journal of Microbiology

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Ortho and meta cleavage dioxygenases detected during the degradation of phenolic compounds by a moderately halophilic bacterial consortium

Abstract

Krishnaswamy Veenagayathri and Namasivayam Vasudevan

Phenolic compounds are carcinogenic and toxic environmental pollutants which are massively discharged to the terrestrial and marine environment from uncontrolled industrial activities. A moderately halophilic bacterial consortium was isolated from different phenol contaminated sites and marine environment, the degradation pathway followed during the degradation of phenol and 4- chlorophenol by the bacterial consortium was investigated. During the degradation of phenol it was found that only catechol 1, 2 dioxygenase activity was pronounced with a maximum specific activity of 0.425μm/min/mg of the protein showing the ortho- cleavage pathway at pH 7. In the degradation of 4- chlorophenol it was found that only catechol 2,3 dioxygenase activity was pronounced with a maximum specific activity of 0.412 μm/min/mg of the protein at pH 7 showing the consortium followed metacleavage pathway during the degradation of the substrate. The cell free extracts of bacterial consortium showed cis-cis muconic acid as the intermediate metabolite during the degradation of phenol and 4- chlorocatechol and 5-chloro-2-hydroxymuconate as intermediates during the degradation of 4- chlorophenol, which proved that the moderately halophilic bacterial consortium followed orthocleavage on phenol degradation and meta-cleavage during the degradation of 4-chlorophenol.

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