Human gut is inhabited by millions of identified and unidentified microbes. It forms an intricate and active, lively and effective population known as the gut microbiota that directly or indirectly affects the hosts’ normal life cycle. It is observed that each organism has a unique microbiota that is in direct interface with the environment. Its composition and diversity is dependent on several factors, that may be any of the, i.e., age, surrounding, life style, food we eat, even with any past medical crisis that we have encountered directly affects the microbiota. It is observed that the microbiota effects the immunological cycle of host. Any change or alteration in the microbiota can led to multiple diseases and can also be the factor for the future related problems, so, it becomes essential to identify the diversity of microbe within the host. As much of the microbes resides in the large intestine, so the human fecal sample being the most appropriate selection for isolation. For identification the 16S rDNA technique has emerged as the most significant one the work. This review summarises our current understanding of the development and composition of the human GI microbiota, its impact on host health aiming to isolate and identify the microbiota using the 16S rDNA sequencing technique.
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