The paper highlights the historic background of Rhizosphere investigations focusing on Root- Soil Interface activities in terms of micro-morphology, using advanced micro-morphological investigation techniques and tools such as high-resolution microscope, scanning electron microscope, electron-probe micro-analyser analysis, X-Ray Diffraction Technique, Thin Micro- Autoradiography using Kodak thin myler films onto thin sections with optical polish < 1.0 micron. The details of the research findings are summarized in the relevant sections of the paper. The second part of the paper refers to a number of factorial pot experiments with French beans (Phaseolus vulgaris L) as test crop, involving three nitrogen sources (viz. ammonium phosphate, choline phosphate and calcium chloride) at 2 N levels (500 ppm n and 1000 ppm N), 2 initial soil pH levels adjusted at pH 7 & pH 8 and two growth (G) stages (21 days and 42 days) were conducted under a standard growth chamber condition (temperature 28°C, light intensity 10, 000 LUX, R. H. 60%). Following dry ashing (475°C) shoots and roots were analysed for H2PO4-2 (Phospho- molybdate method (Jackson, 1964) Ca2+, Mg2+, K+, Na+, NH4+, NO3-, Cl-), dithinate-citrate extractable Fe2+, Mn2+, Zn2+ (Mehra and Jackson, 1960). The analytical values of soil (rhizosphere and non-rhizosphere) and root and shoot parameters were subject to Analysis of Variance Tests (ANOVA Test) to determine significant differences of based on single factors of factorial combinations and reveal ‘main effects’ and interactions. The summary of results of these experiments is briefly summarized in the relevant sections of the paper that follows.
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