International Research Journal of Microbiology

Isolation and screening of antimicrobial producing lactic acid bacteria from fermentating millet gruel

Abstract

Wakil S.M.* and Osamwonyi U.O

The lactic acid bacteria counts, pH and titratable acidity of the fermenting millet gruel were determined using routine methods. The isolated and identified lactic acid bacterial isolates were L. plantarum, L. fermentum, L. meseteriodies, L. jensenii, L. brevis, P. acidilacticiand Lactobacillus spp. These isolates were able to inhibit the growth of various indicator organisms; Escherichia coli, Staphylococcus aureus, Bacillus cereus, Bacillus licheniformis, Salmonella spp., Pseudomonas flourescens, Pseudomonas aeruginosa, Proteus spp., Serratia spp. and Pseudomonas syringae used in this investigation at varying degrees. Quantities of lactic acid, diacetyl and Hydrogen peroxide produced were also determined using standard methods. The pH of the growth medium was observed to have a significant effect on the production of these antimicrobial compounds by the respective lactic acid bacteria.

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