Isolation and in Vitro Antibiotic Susceptibility of Pasteure | 17704
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International Research Journal of Microbiology

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Isolation and in Vitro Antibiotic Susceptibility of Pasteurella multocida from Cattle Origin


Manasa Y. Sugun*, Jasni A. Musa, Moses O. Odugbo, Maryam Muhammad, Elmina Abiyayi and Isa Suleiman

Pasteurellosis is one of the important economic diseases in ruminants, especially in cows and buffaloes. It is caused by Pasteurella multocida and occasionally by Mannheimia haemolytica. During the present study 6 Pasteurella multocida were obtained by cultural, biochemical methods from 26 suspected cases of haemorrhagic septicaemia fron intestinal samples (lungs, liver and spleen) of cattle. Pasteurella multocida species specific specific polymerase chain reaction provides a rapid and specific of all the serotypes of P. multocida and can be used as rapid method for detection P. multocida infection in animal. Two of the isolates were detected to have somatic antigen 3,4 and one isolate 2,5 while in the remaining four, somatic antigens were not detected. Antibiotic susceptibility pattern of the isolates revealed that enrofloxacin, flumequin, Penecillin, chloramphenicol and cephalexin were most susceptible (100%), followed by pefloxacin (93.2%), gentamycin (90.1%), ampicillin (90 %), ciprofloxacin (45.5%), amoxicillin (29.76 %) and erythromycin (18.3%). Where all the P. multocida isolates were found to be resistant against sulphadiazine (100%). The study confirmed the presence of P. multocida with somatic antigen 3, 4 and P. multocida 2, 5 were identified for first time in Gombe sate amongst the isolates. However more work needs to be carried out in other parts of the country to gather more relevant information with regards to somatic types.

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