+44 330 818 7254Tekeba Sisay, Bogale Damtew*, Nega Berhane
Phosphorus (P) is one of the most essential macro-elements vital to increase soil fertility growth and development of plants. However, the occurrences of phosphorus in complex form make it in soluble and in accessible. Hence the objective of the present study was to isolate and characterize phosphate solubilizing bacteria from agricultural field soil sample of Afar region of Dupti wereda, and in south Gondar Zone Kimir-Dingay district Deskua kebele, and to assess their capability to solubilize phosphates This study was conducted from Institute of Biotechnology Department of biotechnology cellular and microbial laboratory in Gondar university. Thus, Phosphate solubilizing bacteria were isolated and characterized morphologically, biochemically. Total of ten phosphate solubilizing bacteria were isolated from the selected site. The isolates were coded as DU1, DU2 DU3, DU4, DU5, DU6, DE1, DE2, DE3 and DE4. From these eight isolates were gram positive and two isolates were gram negative. Their phosphate solubilization efficiency of isolates were identified based on making hole zone or clear zone around PVK media. And liquid broth containing calcium triphosphate. Among the isolates. DU5=6 mm, DE1=6 mm and DE2=6 mm, DU1=5.6 mm have observed to be the maximum solubility index. When solubilization efficiency of isolates observed in liquid broth the highest P release were recorded by isolates DE1=2.16 ± 1.96 mg, DU5=1.01 ± 1.02 mg, DE2=0.907 ± 1.25 mg, DE4=0.7 ± 0.9 mg. Based on solubilization efficiency and stress tolerant capability only six isolates were selected for pot experiment applied in faba bean and wheat variety. Isolate PSB DU5 showed the best effect on shoot length (71.66 ± 15.27), root length (12.66 ± 3.7), leaf number (39 ± 4.35), nod number (13.3 ± 0.57) Isolate PSB DU1 and PSB DE1, DE2 showed second and third best effect on shoot length of Dosha faba bean growth respectively. Similarly, PSB DU3 significantly increased root length When the effect of the isolates is compared based on their shoot length of the inoculated Eliodoro wheat variety, isolate DU1 and DU5 were observed to be the most effective to enhance the plant growth with respect to shoot length.
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