Ghotbi, T. and Shahraeen, N.
During the year 2007-2009 to determine the incidence and distribution of main viral diseases infecting some ornamental plants, samples were collected from ornamental growing glasshouses in Mazandaran and Guilan provinces. Propagated ornamental plants in glasshouses were mostly showing symptoms of systemic necrotic spots and general chlorosis on leaves, growth abnormalities and plant stunting, flower break and flower cup split were also common symptoms. Suspected diseased samples were tested applying routine serological DAS-ELISA, TIBA and Dot-Blot techniques against important diseases of ornamentals, Tomato spotted wilt virus (TSWV), Impatiens necrotic spot virus (INSV), Tomato yellow ring virus (TYRV), Cucumber mosaic virus (CMV), Tomato ring spot virus (ToRSV) and Arabis mosaic virus (ArMV). Selected ELISA positive samples were mechanically back inoculated to specified indicator plants. One the basis of biological and serological reactions the collected ornamental species were identified as a diagnostic natural host for each of the viruses under study. Of the total of 464 ornamental plants (in 23 different families) tested, 224 samples were shown to be infected to the above 6 viruses. On descending order number of infected samples for TSWV were 83 (17.88%), 47 samples for INSV (10.12%), 32 samples for CMV (6.89%), 31 samples for ToRSV (6.68%), 23 samples for TYRV (4.95%), and 8 samples for ArMV (1.72%). Respectively, TSWV and INSV (tospovirus), CMV (cucumovirus) and ToRSV (nepovirus) were the most prevalent viruses infecting ornamental plants in Northern cities of Mazandaran and Guilan province of Iran.
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