Fatima Shujatullah, Indu Shukla, Richa Gupta, Parvez A Khan and Fatima Khan
Infectious diarrheal diseases are responsible for considerable morbidity and mortality, especially in developing countries. The emerging resistance to many antibiotics is a matter of great concern since in the recent past antibacterial resistance in V. cholerae was seldom documented. The emergence of considerable resistance in a short span of time is needed to be addressed, detected, documented and controlled. The present study was done to evaluate the changing pattern of antimicrobial sensitivity pattern in V.cholerae isolated from the stool specimens from diarrhea patients. A total of 386 Stool specimens were processed between 2006 and August 2011 from diarrhea patients admitted to the Jawaharlal Nehru Medical College and Hospital (JNMCH), Aligarh. The specimens were cultured on to MacConkey agar, Thiosulphate-citrate bile salt sucrose (TCBS), Blood agar and inoculated in alkaline peptone water. Isolates were identified as V. cholerae according to the recommended standard laboratory methods and confirmed by serology using antisera. Antimicrobial susceptibility was carried out by Kirby-bauer disc diffusion method. Out of 386 faecal specimens examined from the patients of diarrhea, 66 were positive for V. cholerae. All the isolates during the years 2006 to 2011 depicted a constant increase in resistance to nalidixic acid, ciprofloxacin, tetracyline, doxycycline and cotrimoxazole with 100% resistance to nalidixic acid and cotrimoxazole in some isolates. The isolates remained consistently sensitive to gentamycin, cefotaxime and piperacillin.
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