Jamal Seifdavati, Akbar Taghizadeh
Effect of moist heat autoclave at 127°C with a steam pressure of 117 k Pa for 20 min on in vitro gas production parameters of common vetch (CV), chickling vetch (CLV) and bitter vetch (BV) seeds were evaluated. Gas production volumes were recorded at 2, 4, 6, 8, 12, 16, 24, 36, 48, 72 and 96 h of incubation. The gas production data was fitted using Y = A (1 – e-ct). This experiment was carried out according to a completely randomized design and the data were analyzed using factorial method and the Mixed Model procedure. The obtained data at 24 h after incubation were used for estimation of metabolizable energy (ME), net energy for lactation (NEL), short chain fatty acids (SCFA), digestible organic matter (DOM) and microbial protein (MP). Moist heat decreased (P<0.05) gas production of CV seed after 24 h, but the differences between CLV and BV seeds were not significant. Heating process resulted decreasing in gas production of CV seed after 6 h. The rate of gas production were for CLV (0.052/ h), CV (0.053/ h) and BV seed (0.063/ h) that decreased by moist heat autoclave for CV and BV seeds (P<0.05). Moist heat decreased (P<0.05) gas production of soluble and insoluble fraction (A) of CV seed, but did not affect on CLV and BV seeds. Moist heat of CV seed significantly decreased (P<0.05) values of ME, NEL, SCFA, DOM and MP, but did not affect significantly mentioned parameters in CLV and BV seeds. The rate of gas production of CV seed can be decreased by moist heat autoclave resulting improved rumen ecosystem and decreased risk of health problems due to decreasing of rate of fermentation. The similarity of gas production between CLV and BV seeds indicated the similar fermentation kinetics of their carbohydrate contents.
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