Development and application in Mexican of a method for the i | 17878
International Research Journals

Development and application in Mexican of a method for the identification of polymorphisms of GSTP1


Fernando Mejia-Sanchez, Julieta Cadena-Castillo and Juan Carlos Sánchez-Meza

Glutathione S-transferase (GST) family is formed by several genes coding for a family of enzymes involved in phase II detoxification from xenobiotic s through glutathione conjugation, generating water soluble compounds. GSTs have a pharmacological and toxicological interest due, among other facts, to their increased expression in mammalian tumor cells . GSTP1 is polymorphic, having three allelic variants: GSTP1a wild allele, codon 104 to GTC (Val ) ATC (Ile) GSTP1b, and codon 113 GSTP1c GCG (Ala) to GTG (Val). A procedure was developed for t he identification of polymorphisms by bioinformatic analysis in order to select the primers and enzymes necessary for performing PCR-RFLP, which were BsmAI and AciI. The procedure thus developed was ap plied to 60 Mexican Mestizo healthy individuals, 30 men and 30 women. The resulting frequencies for P1b were P1a/P1a 31.66%; P1a/P1b 59.99% and P1b/P1b 8.33%; while for P1c were P1a/P1a 100%; P1a /P1c and P1c/P1c 0%. These frequencies are obtained in Mexico for the first time.

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