Assessment of In vitro anti-inflammatory activity, phytochem | 84458
International Research Journals

International Research Journal of Plant Science

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Assessment of In vitro anti-inflammatory activity, phytochemical analysis and antimicrobial assay of Tinospora Cordifolia extracts


Pranav Yawalkar*, Ayushree Diyewar, Esheksha Gundre and Prasad M.P

Tinospora cordifolia belonging to the family menispermaceae, is a large extensively spreading glabrous, perennial deciduous twiner with succulent stems and papery bark. In modern medicine, T. cordifolia is used for the treatment of general weakness, fever, dyspepsia, dysentery. Methanol extracts of Tinospora cordifolia leaves was assessed for its anti-inflammatory activity by invitro methods, phytochemical analysis and antimicrobial assay. Invitro anti-inflammatory activity was evaluated using albumin denaturation, proteinase inhibitory activity at different concentrations. The results showed that methanolic extracts significantly Inhibited albumin denaturation, maximum inhibition for soxhlet extract was observed at 300μg/ml and for cold extract was observed at 200μg/ml. For Proteinase Inhibitory Action, it showed maximum inhibition for soxhlet extract at 200μg/ml and for cold extract at 100μg/ml. Phytochemical screening revealed the presence of alkaloids, tannins, cardiac glycosides carbohydrates. Quantitative analysis revealed that total alkaloid content was found to be 5370mg/ml in soxhlet extract and 3990mg/ ml for cold extract, Tannic acid content in soxhlet methanol extract was found to be 0.637 mg/g TAE of extract, Total carbohydrates content in methanol extract (Soxhlet) = 1.014 mg/ml total carbohydrate content in methanol extract (Cold) = 0.610 mg/ml and cardiac glycosides showed the absorbance of 0.06 and transparency was 88 % when measure for soxhlet methanol extract. The antibacterial assay of the extracts was studied using agar well diffusion method against certain isolates. Results suggest that the cold extract showed some activity against tested bacteria whereas, soxhlet extract showed no activity against tested bacteria.

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