Anti-inflammatory and anti-nociceptive effects of crude extr | 16749
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International Research Journal of Pharmacy and Pharmacology

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Anti-inflammatory and anti-nociceptive effects of crude extract and fractions of Morinda morindoides root bark


Olukunle J.O, Abatan M.O, Adenubi O.T, Oyebanji B.O and Ogbole O.O

This study was carried out to assess the anti-inflammatory and anti-nociceptive effects of the extract of Morinda morindoides root bark to confirm folkoric claims. Anti-inflammatory and analgesic effects of the methanol extract, solvent fractions and Chromatographic sub-Fractions (CsF) A-F of the bioactive fractions of Morinda morindoides root bark were determined using carrageenan induced rat paw oedema model, thermal and acetic-acid induced writhing method in mice. Extracts of Morinda morindoides root bark at 400, 200 and 100mg/kg of methanol, solvent fractions (hydro methanol, hexane, chloroform and ethyl acetate) and bioactive CsF of the root bark were assessed in rats, which were compared with controls; a negative control given 10ml/kg Tween 80, positive control groups administered with Indomethacin, aspirin, paracetamol and morphine. The mean percentage inhibition of paw volume was highest in rats to which indomethacin was administered (85.65%), followed by rats administered aspirin (68.40%) which was comparable with that of rats dosed with 400mg/kg of Morinda morindoides root extract (65.52%).While the chloroform fraction and chloroform CsF A of M. morindoides at 200, and 100mg/kg respectively showed bioactivity with the highest percentage inhibition of increase in paw volume (93%). Also, extract increased significantly (P<0.05) the reaction time in the hot plate test (26.0+0.7)s and the mean number of writhing was significantly lower (P<0.05) in groups dosed with 400mg/kg methanol extract and 100mg/kg of ethyl acetate CsF D of Morinda morindoides root bark extract. The study establishes the anti-inflammatory and anti-nociceptive potential of M. morindoides root bark extract in methanol, chloroform and ethyl-acetate solvents.

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