+44 330 818 7254Olukunle J.O, Abatan M.O, Adenubi O.T, Oyebanji B.O and Ogbole O.O
This study was carried out to assess the anti-inflammatory and anti-nociceptive effects of the extract of Morinda morindoides root bark to confirm folkoric claims. Anti-inflammatory and analgesic effects of the methanol extract, solvent fractions and Chromatographic sub-Fractions (CsF) A-F of the bioactive fractions of Morinda morindoides root bark were determined using carrageenan induced rat paw oedema model, thermal and acetic-acid induced writhing method in mice. Extracts of Morinda morindoides root bark at 400, 200 and 100mg/kg of methanol, solvent fractions (hydro methanol, hexane, chloroform and ethyl acetate) and bioactive CsF of the root bark were assessed in rats, which were compared with controls; a negative control given 10ml/kg Tween 80, positive control groups administered with Indomethacin, aspirin, paracetamol and morphine. The mean percentage inhibition of paw volume was highest in rats to which indomethacin was administered (85.65%), followed by rats administered aspirin (68.40%) which was comparable with that of rats dosed with 400mg/kg of Morinda morindoides root extract (65.52%).While the chloroform fraction and chloroform CsF A of M. morindoides at 200, and 100mg/kg respectively showed bioactivity with the highest percentage inhibition of increase in paw volume (93%). Also, extract increased significantly (P<0.05) the reaction time in the hot plate test (26.0+0.7)s and the mean number of writhing was significantly lower (P<0.05) in groups dosed with 400mg/kg methanol extract and 100mg/kg of ethyl acetate CsF D of Morinda morindoides root bark extract. The study establishes the anti-inflammatory and anti-nociceptive potential of M. morindoides root bark extract in methanol, chloroform and ethyl-acetate solvents.
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