Ayyub Patel, Elsayed Hafez, Fahmy Elsaid, Mohammed Amanullah
The role of a new lectin protein from Acacia specie s found in Aseer region of Kingdom of Saudi Arabia, as anti-cancer agent on four cell lines viz. HepG-2 (Hepatocellular carcinoma), MCF-7 (Breast cancer), HCT 116 (colon Cancer) and HEP-2 (Larynx c ancer) was evaluated. Acacia seyal del , was collected from Aseer region of the Kingdom of Saudi Arabia. The plant tissue was subjected to RNA extraction, the extracted RNA was used for lectin g ene amplification using specific primers. Cloning, subcloning of the Acacia 400bp gene was carried out and in vitro transcription, combined with protein purification was undertaken. The cytotoxicity of th e recombinant lectin was performed on HepG-2 (Hepatocellular carcinoma), MCF-7 (Breast cancer), HCT 116 (colon Cancer) and HEP-2 (Larynx cancer). Our study resulted in the observation of t wo amplicones with molecular sizes of 800 and 400bp. The 400bp amplicone was excised from the agr ose gel, purified and sequenced. The sequence analysis revealed that the lectin gene isolated fro m Acacia seyal del showed similarity with Lotus japonicus nod factor binding lectin gene with an identity of 95%. The purified lectin exhibited a significant cytotoxic effect on MCF7cell. The IC 50 value for HepG-2 cells was 100 μg/ml and for MCF7 was 250 μ g/ml interposed from linear regression curve. Thus we conclude that Acacia lectin has anti- cancer action at different concentrations especiall y at a high dose 100 μg/ml.
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