Analysis of few crude compounds and separation of leaf pigme | 69010
International Research Journals

International Research Journal of Plant Science

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Analysis of few crude compounds and separation of leaf pigments in few medicinal plant species


Ranjith N.P1, Noorbina E, Veerabhadra Swamy A.L* and Mallikarjunswamy K.S

For utilization of a certain biomass it is necessary to know its chemical composition. The present study is to qualitative crude chemical analyses of nutritional value (crude compounds) such as acidity, starch, carohydrates, iron and calcium etc. from stem extracts of Tinospora cardifolia, Centella asiatica, Clerodendrum inerme and leaf extract of Ocimum sanctum, Lawsonia inermis and Piper nigrum were conducted and also separate the pigments by TLC methods. The results revealed that Tinospora cardifolia, biomolecules like starch and carbohydrates were present and elements like iron and calcium were absent. In Centella asiatica indicated the absence of biomolecules like starch and carbohydrates and only one molecules i.e., calcium is present and iron is absent. Similarly, in Clerodendrum inerme, biomolecules like starch were absent and carbohydrates were present. Both the elements iron and calcium were absent in this plant extracts. In Ocimum sanctum, starch is absent carbohydrates are present, iron and calcium are absent. In Lawsonia inermis, the results are similar to Ocimum sp. i.e., only carbohydrates are present remaining starch, iron and calcium are absent in this leaf extracts. Interestingly, in Piper nigrum, all chemical compounds are totally absent. Related to pigments analysis highest distance travelled pigments are carotenoids (4.3 cm) in O. sanctum and highest number of pigments was found in C. inerme with six pigments and least number of pigments was recorded in O. sanctum (four) and L. inermis (four). Meanwhile, highest Rf value recorded in O. sanctum and P. nigrum with 0.97 (chlorophyll a). Among the seven pigments in six species carotenoids, chlorophyll a and xanthophylls are common to all species but chlorophyll b, lutein, anthoc

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