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Allelopathic potentiality of two Heliotropium species on ger | 16403
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International Research Journal of Biotechnology

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Allelopathic potentiality of two Heliotropium species on germination and protein expression of some plants

Abstract

Hussein F. Farrag, Abdallah M. Sliai and Tha´ar F. Mhmas

In this study we compare the allelopathic potentiality of two invasive species; Heliotropium curassavicum and H. bacciferum on germination, seedling growth and protein expression of Calotropis procera, Faba sativa and Lycopersicon esculentum. The germination of C.procera, F.sativa and L.esculentum controls was 100%, and inhibition of this germination increased with increasing the extract concentrations. Minimum germination values were recorded at the highest extract concentration (T3) and amounting to 20, 52 and 76% for C.procera, F.sativa and L.esculentum plants treated with H. curassavicum and were 52, 72 and 60% for the same treatment and test plants but treated with H. bacciferum extract. Values of plumule lengths were generally higher than that of radicle in all test species using either of the two invasive species extract. Radicle-plumule ratios for all test species and using any of the two extracts were less than unity except control F.sativa plants which recorded 1.14. Dry matter allocation toward plumule is generally higher than that of radicle in all test plant treatments except for one case; L.esculentum control which recorded allocation amounting to 44.83% for plumule and 55.17% for radicle. Expression of proteins in treated plants of the present study was significantly increased or decreased at the level of number and intensity of protein bands as compared to control plants, depending on the type and concentration of extract treatment. According to the unweighted pairgroup arithmetic mean method (UPGMA) dendrogram, the expressed proteins of control plants were the farthest among the different treatments of the three test plants.

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