+44 3308180992Eshwar Singh*, DK Rana, Himani Rana, Himani Rawat
Vegetables are a crucial part of agricultural production systems and play a vital role in sustaining human life. Various biotic and abiotic stresses pose a threat to the growth, yield and quality of these crops. Addressing these challenges is essential for ensuring food security and agricultural sustainability. While conventional breeding techniques have greatly advanced the development of key varieties, new methods are needed to further enhance horticultural crop production. Recent plant breeding tools such as the CRISPR/Cas9 technique offer rapid, cost-effective and precise methods for crop improvement. CRISPR-associated protein 9 (Cas9) has proven to be a valuable genome-editing tool, capable of altering DNA sequences at specific loci with precision. With access to whole-genome sequencing data and knowledge about gene functions for important traits, CRISPR-Cas9 editing can precisely mutate key genes. This capability allows for the rapid generation of new germplasm resources to enhance important agronomic traits. This review provides an in-depth overview of CRISPR-Cas9 gene editing technology and explores its potential applications in olericulture as well as the challenges it faces.
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