An antigen that can trigger an immunological response is known as an immunogen. The production of antibodies is reliant on a humoral immune response, which is carried out by immune cells that identify a molecule as foreign. When an immunogen is injected alongside an adjuvant, the host's immune system is pushed to mount a particular immunological response, producing antibodies against the immunogen. Antigens less than 20 kDa (200 amino acids) are often not immunogenic. They must be attached to a carrier protein. The concentration of the injected antigen has an impact on immunogenicity in addition to overall size (Carter et al., 2001). The inoculation volume has to be more concentrated the lower the antigen's immunogenicity
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