K. Cḫ̩rifi1, H. Boubaker1, F. Msanda1, B. Saadi1, E. Boufous2 and A. El Mousadik1
Variability of salt tolerance in eight wild populations of two annual Medicago species (Medicago ciliaris and Medicago polymorpha) was evaluated at germination stage using three treatments of salinity: 50, 100 and 150 mM of NaCl. Results showed that germination is clearly affected by high salt stress; their germination rate does not exceed 7% at 150 mM. The greatest variability in tolerance was observed at moderate salt stress (50 mM of NaCl) and the decrease in germination seems to be more accentuated in M. polymorpha than M. ciliaris, whereas, the Tunisian population of M. ciliaris was the more tolerant in all Moroccan ecotypes studied in this work. This population prospected on soils affected by salinity exhibits a particular adaptability to salt environment, at least at this stage in the life cycle. This intraspecific variation in salt tolerance may be used to select genotypes particularly suitable for cultivation on lands relatively affected by salinity. On the other hand, when ungerminated seeds from NaCl treatments were transferred to distilled water, they recovered largely their germination at all the populations studied after only 2 days. This indicates that the germination inhibition was related to osmotic stress rather than ion toxicity. In addition, seed germination in all populations tended to be extremely rapid than that observed in distilled water which indicates that this pretreatment raises dormancy.
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