International Research Journal of Biotechnology

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Silver nanoparticles damage yeast cell wall


Debabrata Das and Giasuddin Ahmed

Silver nanoparticles are increasingly finding applications in health care and consumer products. Although the bactericidal properties of silver nanoparticles are well documented, there is scant data available on their effect on eukaryotic cells. We report results from experiments concerning the model organism Saccharomyces cerevisiae. A novel method of nanoparticle synthesis in yeast malt media was achieved. Characterization of particles by electron microscopy and XRD revealed a mean particle size of 8.6 nm. Micrographs of cells treated with nanoparticles revealed deposition on and extensive damage to cell wall/membranes. Observations on membrane damage were confirmed by an in vitro cell permeability assay.

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