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International Research Journal of Plant Science

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Shoot Proliferation Using Cotyledonary Nodes of Locally Conserved Parkia biglobosa (Jacq.) Benth Seeds

Abstract

Abdullahi, I. N and Akinyele, A. O

The study was conducted to establish a protocol for in vitro multiplication of shoots from cotyledonary nodes obtained from locally conserved seeds of Parkia biglobosa for genetic improvement and germplasm conservation. The objectives include determination of plant hormone that induces shoot proliferation and the explant that shows rapid shoot multiplication. Axillary and apical nodes of 8 and 12 weeks old seedlings of P.biglobosa were used as explants on Woody Plant Medium (WPM). NAA, KIN and BAP were supplemented at 0.0, 0.5, 0.75, 1.0 mg/l for inducing shoot. Parameters measured include number and length of shoots and the number of nodes of the cultured explants. The design is a 2x2x3x4 factorial experiment in a Complete Randomized Design (CRD). Data obtained were analysed using Analysis of Variance and significant means were separated using Least Significance Difference (LSD) at 0.05 and 0.01 respectively. It was observed that for 8 weeks old axillary node, shoot length increases as the concentration of hormone. Age and age-hormone interaction had significant effect on the number of nodes (P=0.05) with the highest mean, 7 obtained among 8 weeks apical nodes of 0.75mg/l and 1.00mg/l KIN. It was observed that organogenesis using juvenile cuttings would be effective for this species.

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