International Research Journal of Biotechnology

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Rhizome derived callogenic plantlet production of Mantisia wengeri (Zingiberaceae), a rare and endemic medicinal plant of Mizoram, North-East India

Abstract

Sudipta S. Das Bhowmik, Suman Kumaria and Pramod Tandon

Mantisia wengeri Fischer is a rare and endemic zingiber of North-East India which has been traditionally used for curing bone fracture and gastro-intestinal ailments. However due to the extreme rarity of the germplasm, studies related to genetic diversity, phytochemical properties etc. in this plant has not been feasible. The study describes the development of high efficiency regeneration protocol for obtaining large- scale diverse plantlets from rhizomatous callus tissues in Murashige and Skoog medium incorporated with various concentration of growth regulators N6-benzyladenine (BA), αNapthalene acetic acid (NAA), 2, 4-dichlorophenoxyacetic acid (2,4-D) and sucrose at elevated concentrations. Bud forming capacity (BFC) to a maximum of 33.5±3.7 was recorded from the organogenic callus tissues of M. wengeri within 8 - 10 weeks in MS medium supplemented with a combination of 10µM of BA and 40µM NAA at 10% sucrose. The callogenic plantlets are been transferred to the field for studying morphological and genetic variations.

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