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International Research Journal of Plant Science

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Regeneration and analysis of genetic stability of plantlets as revealed by RAPD and AFLP markers in date palm (Phoenix dactylifera L.) cv. Deglet Nour

Abstract

A. Othmani, S. Rhouma, C. Bayoudh, R. Mzid, N. Drira and M. Trifi

The 2,4-Dichlorophenoxyacetic acid (2,4-D) induced somatic embryogenesis of Tunisian date palm (Phoenix dactylifera L.) cultivar, Deglet Nour and analysis of the true-to-type conformity of the derived plantlets were investigated in this study. For this purpose, two polymerase chain reaction (PCR)-based methods namely, randomly amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) markers were used. Data proved that the modified Murashige and Skoog (MS) media including 1, 10 and 100 mg.l-1 2,4-D have permitted an intensive callogenesis when leaves are incubated in dark. Subcultures on MS medium supplemented with 0.1 mg.l-1 2,4-D stimulated a rapid maturation of somatic embryos in light. A mean of 120 somatic embryos were developed from 0.5 mg callus within 3 months. Embryos germination and conversion to plantlets were successfully achieved after transfer to free plant growth regulators MS medium. On the whole, 75% plantlets survival was established in soil. In addition, RAPD and AFLP analysis were performed in 180 randomly selected plantlets. The resultant DNA banding profiles exhibited a genetic stability of regenerated plantlets compared to the plants of origin. This result strongly supported the true-to-type nature of the in vitro derived plants in date palm.

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