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Presence of Atypical Antibodies in a Previously Transfused Population in Jos Metropolis| Abstract

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Presence of Atypical Antibodies in a Previously Transfused Population in Jos Metropolis

Abstract

Tokdung M, Ogwuche S.A.R, Sabo A. M and Odeh S.O

200 blood samples were collected from previously tr ansfused participants who gave their informed consents (age between 5 – 60 years) after at least six months post transfusion, to determine the presence of atypical (immune) antibodies. The number of pint of blood received was also taken into consideration. 137 (68.5%) of the subjects had receive d 1 pint of blood, 22 (11.0%) of them had received 2 pint of blood and 41 (20.5%) had received more than 2 pint of blood. The antibody screening (at saline room temperature, saline 37 o C, albumin and antihuman globulin) showed that the group that received 1 pint had 63.50% (87 samples out of the total of 137 samples), while recipients of 2 pint of blood had 68.18% (15 samples out of a total of 22 samples) and reci pients of more than 2 pint of blood had 78.05% (32 samples out of a total of 41 samples). The cumulative prevalence is 68.0% (134 samples out of 200 samples). At p<0.05 statistical level of significance, 5.81 was obtained, as against the tabulated value of 9.55. It was also observed that none of the samples reacted at room temperature. Furthermore, recipients of 2 pint and above reacted at saline 37 o C; albumin and antihuman globulin, only 55.17% (48 samples out of 87 samples) reacted at saline 37 o C and all of them reacted with albumin and antihuman globulin (p<0.05). It is concluded that all the anti bodies were immune, warm and incomplete in nature, confirming the presence of atypical antibodies in previously transfused persons

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