Amir Jalili, Asra Sadeghzade, Shahriar Alipour
Walnut (Juglans regia L.) leaves from eleven different cultivars (Mayette, Fernor, Mellanaise, Elit, Orientis, Lara, Hartley, Franquette, Parisienne, Arco, Marbot) were studied for their phenolic compounds.The evolution of major phenolic compounds amounts was monitored from May to September. Two extractive procedures were assayed and the best results were obtained using acidified water (pH 2) and a solid phase extraction column purification step. Qualitative analysis was performed by HPLC-DAD/MS and, in all samples; nine phenolic compounds were identified (3- caffeoylquinic, 3-p-coumaroylquinic and 4-p-coumaroylquinic acids, quercetin 3-galactoside, quercetin 3-arabinoside, quercetin 3-xyloside, quercetin 3-rhamnoside, quercetin 3-pentoside and kaempferol 3-pentoside). Quantification of phenolic compounds was performed by HPLC-DAD, which revealed that the highest content of phenolics was found in May and July and quercetin 3- galactoside was always the major compound while 4-p-coumaroylquinic acid was the minor one.
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