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International Research Journal of Microbiology

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Optimization of environmental and nutritional conditions to improve growth and antibiotic productions by Streptomyces Sp. Isolated from Saudi Arabia Soil

Abstract

Ibtisam M Ababutain, Zeinab K Abdul Aziz and Nijla A AL-Meshhen

The purpose of the present study was to optimize the environmental and nutritional conditions for the isolate Streptomyces sp. MS-266 Dm4 to improve antibiotic production. The results showed that the highest antimicrobial activity were obtained after 7 days of growth under shaking conditions at initial pH 6 and temperature 30°C. Starch (2g/100ml) was found to be the most suitable carbon sole source for optimum growth and antibiotic productions. Peptone (0.04g/100ml) and NaNO3 (0.2g/100ml) were found to be the most suitable nitrogen sole source for antibiotic productions and optimum growth, respectively. The effect of different concentration of mineral salts, were investigated. The maximum antibiotic production by Streptomyces sp. MS-266 Dm4 was obtained in a medium of the following composition (g/l): Soluble starch 2.0, peptone 0.04, K2HPO4 0.2, MgSO4.7H2O 0.15, KCL 0.05 and FeSO4.5 H2O traces, at pH 6.0 incubation period was seven days under shaking conditions.

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