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International Research Journal of Microbiology

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Molecular detection of Candida krusei

Abstract

Anna Brillowska-D├â┬â├ó┬Ç┬×├â┬é├ó┬Ç┬Žbrowska* and Aleksandra Siniecka

The species identification of fungi belonging to genus Candida is an important issue as this genus becomes the emerging problem of nosocomial infections. As Candida krusei presents intrinsic resistance to the fluconazole that is the drug of the first choice in case of invasive candidiasis the PCR identifying the DNA of C. krusei was elaborated. The analytical sensitivity of the assay on spiked blood samples was estimated at 3-5 CFU/ml. The possibility of performing the reaction in both end-point and real-time detection format broadened the potential of its application in small and medium microbiology laboratories.

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