GET THE APP

Isolation, purification and mass production of protease enzyme from bacillus subtilis| Abstract

süperbetin

International Research Journal of Microbiology

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Isolation, purification & mass production of protease enzyme from bacillus subtilis

Abstract

Gitishree Das* and M.P. Prasad

Screening and isolation of protease producing strains of bacteria were carried out from four different soil samples collected from various places in Bangalore. The isolates were positive on skim milk agar (1%) and thus are selected as protease producing strain. The organisms were tested for various biochemical tests, which lead to their identification as Bacillus subtilis producing protease enzyme. These Bacillus subtilis could group upto 40ºC and pH range 6-9 with optimal growth temperature and pH at 37ºC and 8.0 respectively. It was also optimized for carbon test and nitrogen test with optimal growth in dextrose and peptone respectively. Enzyme production was carried in 1 litre of optimized media in the fermenter at 37ºC for 48 hours at pH 8.0. Harvested protease product was purified by salt precipitation method. Finally the enzyme protease was purified by column chromatography. The protein was characterized using SDS-PAGE. This result showed that Bacillus subtilis under study is a good producer of extra cellular protease, which can be beneficial for industries.

Share this article

tempobet

tempobet girişbursa escort

tempobet giriş

tempobet giriş linki tipobet e yeni giriş tipobet e yeni giriş tipobet son giriş süpertotobet güncel